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1) If you use the Twitter REST API:
You can try to use some "query operators", as defined in the Twitter REST API documentation: https://dev.twitter.com/rest/public/search Some are not listed but you can find them running an advanced search on the website: https://twitter.com/search-advanced
Selecting users with more than x followers is not an available option.
2) If you use the Twitter Streaming API (used by the DSS built-in connector):
Options look more limited.